Esterol 14-demetilase

Esterol 14-demetilase
Indicadores
Número EC	1.14.13.70
Bases de dados
IntEnz	IntEnz
BRENDA	BRENDA
ExPASy	NiceZyme
KEGG	KEGG
MetaCyc	via metabólica
PRIAM	PRIAM
Estruturas PDB	RCSB PDB PDBe PDBj PDBsum
Gene Ontology	AmiGO / EGO
Pesquisa
PubMed Central	artigos
PubMed	artigos

Esterol 14-demetilase é uma enzima que catalisa a reação química: obtusifoliol + 3 O₂ + 3 NADPH + 3 H⁺ $\rightleftharpoons$ 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formiato + 3 NADP⁺ + 4 H₂O.

Os quatro substratos desta enzima são o obtusifoliol, O₂, NADPH, e H⁺, e os quatro produtos são 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol, formiato, NADP⁺, e H₂O.

Esta enzima pertence a família das oxidoredutases e participa na biossíntese dos esteroides.

Referências

Bak S, Kahn RA, Olsen CE, Halkier BA (1997). «Cloning and expression in Escherichia coli of the obtusifoliol 14 alpha-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14 alpha-demethylases (CYP51) from fungi and mammals». Plant. J. 11 (2): 191–201. PMID 9076987. doi:10.1046/j.1365-313X.1997.11020191.x
Aoyama Y, Yoshida Y (1991). «Different substrate specificities of lanosterol 14a-demethylase (P-45014DM) of Saccharomyces cerevisiae and rat liver for 24-methylene-24,25-dihydrolanosterol and 24,25-dihydrolanosterol». Biochem. Biophys. Res. Commun. 178 (3): 1064–71. PMID 1872829. doi:10.1016/0006-291X(91)91000-3
Aoyama Y, Yoshida Y (1992). «The 4 beta-methyl group of substrate does not affect the activity of lanosterol 14 alpha-demethylase (P-450(14)DM) of yeast: difference between the substrate recognition by yeast and plant sterol 14 alpha-demethylases». Biochem. Biophys. Res. Commun. 183 (3): 1266–72. PMID 1567403. doi:10.1016/S0006-291X(05)80327-4
DHR (1972). «The removal of the 32-carbon atom as formic acid in cholesterol biosynthesis». J. Chem. Soc. Chem. Commun.: 383–385