Proteoma

The proteome is the collection of proteins found in a particular cell type under a particular type of stimulation. It is very roughly the protein equivalent of the genome.

O proteoma é a colecção de proteínas de uma determinada célula que está sob um determinado tipo de estímulo. É o equivalente proteico do genoma.

Proteomics has largely been practiced through the separation of proteins by two dimensional gel electrophoresis. In the first dimension, the proteins are separated by isoelectric focusing, which resolves proteins on the basis of charge. In the second dimension, proteins are separated by molecular weight. The gel is dyed with Coomassie Blue or silver to visualize the proteins. Spots on the gel are proteins that have migrated to specific locations.

O proteoma tem sido estudado tem sido estudado pela separação das proteínas por electroforese bidimensional. Na primeira dimensão, as proteínas são separadas em função da sua carga. Na sefgunda dimensão, as proteínas são separadas com base na massa molécular. É utilizado um corante para visualizar as proteínas. As marcas no gel são proteínas que migraram para uma localização específica.

The mass spectrometer has augmented proteomics. Mass mapping identifies a protein by cleaving it into short peptides and then deduces the protein's identity by matching the observed peptide masses against a sequence database. Tandem mass spectrometry, on the other hand, can get sequence information from individual peptides by isolating them, colliding them with a nonreactive gas, and then cataloging the fragment ions produced.